Technology for Sensitive Multiplexed Protein Detection

Background

Many sensitive assays are available for the measurement of DNA and RNA. By contrast, proteins are currently difficult to quantify. Existing test methodologies such as ELISA do not provide the desirable characteristics of sensitivity, low cost, speed, and parallel analysis.

Description

DNA/RNA assays can achieve exquisite sensitivity by multiplying the analyte via the PCR reaction until a detection threshold is reached. No such similar technology exists for proteins. Biodesic has developed a patented molecule that transduces one detection event into a large measurable signal. This transduction permits sensitivity to the presence of even a single molecule in solution — five orders of magnitude better than traditional ELISAs. Quantitative measurements can be obtained by fixing the transduction coefficient at a given value and measuring the resultant signal.

Beyond sensitivity the technology provides several other important benefits. The fluid-phase nature of the assay ensures maximum biological reactivity and minimum test-induced artifacts. Parallel analysis of multiple analytes in a single sample is possible as the Biodesic methodology allows for a simple de-multiplexing operation after measurement. Finally, total measurement costs are small. The technology is backward compatible with existing laboratory infrastructure, which eliminates large capital outlays, and the process permits the use of crude samples such as whole blood and saliva, which reduces sample preparation time and expense.

Advantages

• Ability to quantify proteins
• Single molecule sensitivity
• Parallel measurement of multiple targets
• Low training, sample preparation, and equipment costs

Applications

• Medical Diagnostics
• Drug Discovery
• Homeland Security
• Environmental Monitoring (food/water safety)
• Basic Research

Inquiries

Dr. Rob Carlson
Email: bizdev at biodesic.com